Anthropogenic habitat modification alters calling phenology of frogs.

Anthropogenic habitat modification significantly challenges biodiversity. With its intensification, understanding species' capacity to adapt is critical for conservation planning. However, little is known about whether and how different species are responding, particularly among frogs. We used a continental-scale citizen science dataset of >226,000 audio recordings of 42 Australian frog species to investigate how calling-a proxy for breeding-phenology varied along an anthropogenic modification gradient. Calling started earlier and breeding seasons lengthened with increasing modification intensity. Breeding seasons averaged 22.9 ± 8.25 days (standard error) longer in the most modified compared to the least modified regions, suggesting that frog breeding activity was sensitive to habitat modification. We also examined whether calls varied along a modification gradient by analysing the temporal and spectral properties of advertisement calls from a subset of 441 audio recordings of three broadly distributed frog species. There was no appreciable effect of anthropogenic habitat modification on any of the measured call variables, although there was high variability. With continued habitat modification, species may shift towards earlier and longer breeding seasons, with largely unknown ecological consequences in terms of proximate and ultimate fitness.

Species' traits drive amphibian tolerance to anthropogenic habitat modification.

Anthropogenic habitat modification is accelerating, threatening the world's biodiversity. Understanding species' responses to anthropogenic modification is vital for halting species' declines. However, this information is lacking for globally threatened amphibians, informed primarily by small community-level studies. We integrated >126,000 verified citizen science observations of frogs, with a global continuous measure of anthropogenic habitat modification for a continental scale analysis of the effects of habitat modification on frogs. We derived a modification tolerance index-accounting for anthropogenic stressors such as human habitation, agriculture, transport and energy production-for 87 species (36% of all Australian frog species). We used this index to quantify and rank each species' tolerance of anthropogenic habitat modification, then compiled traits of all the frog species and assessed how well these equipped species to tolerate modified habitats. Most of Australia's frog species examined were adversely affected by habitat modification. Habitat specialists and species with large geographic range sizes were the least tolerant of habitat modification. Call dominant frequency, body size, clutch type and calling position (i.e. from vegetation) were also related to tolerance of habitat modification. There is an urgent need for improved consideration of anthropogenic impacts and improved conservation measures to ensure the long-term persistence of frog populations, particularly focused on specialists and species identified as intolerant of modified habitats.

Maternal Temperature, Corticosterone, and Body Condition as Mediators of Maternal Effects in Jacky Dragons (Amphibolurus muricatus).

AbstractTemperature is a crucial environmental component that imposes physiological constraints and ultimately produces variation in life-history traits. Temperatures experienced by mothers can influence offspring phenotypes, including growth and sex ratios, especially in ectothermic species. However, mechanisms by which thermal information can be passed onto offspring have been underexplored. Here, we investigated corticosterone as a potential mediator of thermal maternal effects. We held female jacky dragons (Amphibolurus muricatus) in two different thermal regimes (short [7 h] or long [11 h] basking treatments), then quantified plasma corticosterone levels and tested for correlations between the resulting corticosterone levels and reproductive outputs. Lizards in the long-bask treatment had significantly higher corticosterone levels than those in the short-bask treatment. Maternal corticosterone, in turn, had sex-dependent effects on offspring hatching size but was not associated with maternal reproductive effort or offspring sex or growth. In contrast, maternal body condition was strongly positively related to both reproductive output (including clutch size and total number of eggs) and offspring size at hatching but had no effect on offspring growth. Basking treatment also interacted with condition and corticosterone to affect egg mass and hatchling snout-vent length, respectively. When we tested for relationships between corticosterone levels and body condition, we found corticosterone to be negatively related to condition in long-bask lizards but only in the postbreeding season. These findings indicate that thermal opportunity alters physiology, with potential consequences for fitness. Moreover, the results suggest interactive influences of temperature, corticosterone, and condition as mediators of maternal effects.